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Presentation of the Wivitis project at the 16th scientific days of the Réseau Francophone de Fluxomique et de Métabolomique


As part of the 16th scientific days of the « Réseau Francophone de Fluxomique et de Métabolomique» (RFMF, June 4-6, 2024, Saint-Malo, France), Olivia Ledieu (INRAE Colmar) presented a poster detailing her work as part of the Wivitis project, on the study of grape cuticular lipids by high-resolution mass spectrometry. Discussions with participants led to new avenues for the identification of compounds present in grape cuticles.
Presentation of the Wivitis project at the 16th scientific days of the Réseau Francophone de Fluxomique et de Métabolomique
Photo: INRAE




Started: Prototype for the simulation of heat stress in grapevines


The INRAE center of Colmar, Alsace, takes in charge a part of the WiVitis project centered on developing a device capable to provoke a thermic stress on grapevine. The basic idea is to be able to simulate an extreme climatic event (a heatwave) with an accuracy never reached before in agronomic studies. By simulating a heatwave, the researchers aim to explore the potential effects of heat on PIWI varieties’ resistance to pathogens.

Researchers of both SVQV (Santé de la Vigne et Qualité du Vin: grapevine health and wine quality) and UEAV (Unité Expérimentale Agronomique et Viticole: agronomic and viticultural experimental unit) units, guided by Łukasz Tarkowski, are developing a prototype for a heating device based on the use of infrared radiators. The prototype building and establishment is a key step to understand which parameters should be used for the final device in order to avoid construction mistakes.

As now, the prototype is almost finished and connectivity test are ongoing. Once the connectivity is validated, infrared radiators will be added to the prototype to make the first heat stress simulations.

Stay tuned!

Blatttemperatursonden
Shown are leaf temperature probes used in the prototype of the thermic stress device and installed on grapevine leaves. The probes have two temperature sensors, perpendicular to each other to allow simultaneous measure of air and leaf temperatures. | Foto: INRAE

Selection des stress
Source: Łukasz Tarkowski, INRAE





Project meeting
Intensive exchange of the INTERREG VI project network WiVitis on 10 April 2024


The annual WiVitis project meeting took place this year at the Julius Kühn Institute (JKI) in Siebeldingen and was attended by (almost) all project partners from Germany, France and Switzerland. As the WiVitis network has only been fully staffed for a few months, all partners involved got to know each other personally and presented their methods and first results. Data collection strategies for the 2024 season were finalised, experimental set-ups discussed and protocols agreed. This is of particular importance, as comparable data sets are collected at the various research sites in the Upper Rhine region and used for cross-site variety characterisation. The PIWI grape varieties being comprehensively analysed in the project include Souvignier Gris, Calardis Blanc, Cabernet Blanc and Floreal.

The meeting began with a brief administrative report by the project coordinator Dr Katja Herzog, who illustrated the methods established at the Institute for Grapevine Breeding Geilweilerhof (JKI) for the high-throughput collection of objective data sets on grape and berry characteristics. The scientists at the JKI Institute will also use these in WiVitis to characterise varieties on the basis of their grape architecture and berry skin surface. These descriptive trait data (= phenotyping data) form the basis for estimating the Botrytis resistance of grape varieties and for developing molecular markers, which are used in vine breeding for the early selection of resistant seedlings. The State Viticulture Institute Freiburg (WBI) impressively demonstrated the infection prognoses for grapevine peronospora for PIWI grape varieties, which were further developed in the VITIFIT project and will be available to the whole of viticulture practice in the online prognosis tool VitiMeteo. In WiVitis, these infection prognoses for PIWI grape varieties will now also be developed for powdery mildew and corresponding tests realised. Microscopic analyses complement these tests and provide insights into the course of infection with the aim of deriving recommendations for action. Infection tests for powdery mildew and botrytis bunch rot and their effects on selected PIWI and traditional grape varieties are also being carried out by the Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz in WiVitis. The question of how Botrytis strains, which have been characterised as differently aggressive, affect the course of infection of Botrytis-resistant PIWIs remains exciting.

Wivitis project meeting at april 10th 2024
Group photo from front left to back right: René Fuchs (WBI), Annika Ziehl (JKI), Katja Herzog (JKI), Alessandra Maia-Grondard (INRAE), Reinhard Töpfer (JKI), Elke Herrmann (DLR), Noemi Flubacher (WBI), Olivia Ledieu (INRAE), Ruth Walter (DLR), Evi Bieler (NILab), Lukasz Tarkowski (INRAE), Raymonde Baltenweck (INRAE), Marcus Wyss (NILab), Bea Steinemann (FiBL), Philippe Hugueney (INRAE). Missing: Hans-Jakob Schärer (FiBL) | Photo: Julia Fuchs, JKI

INRAE Grand Est Colmar reported on the current status of the analytical analyses of the lipid composition on the berry skin surface (= wax layer) of four PIWIs and two traditional varieties. The method used for this was adapted and newly established for berries in WiVitis. Initial results showed variety-specific differences in the lipid compositions detected, and these results are to be further consolidated in 2024 using samples from different locations. The partners in Colmar are also setting up a test facility to treat potted vines with heat stress and to investigate the effects of massively rising temperatures, such as those that can be found during heatwaves, on powdery and downy mildew infestation.

The Swiss partners from the Research Institute of Organic Agriculture (FiBL) aim to collect data in organically farmed vineyards and reported on sampling for investigations into the lipid composition on the surface of the berry skin and development and infection analyses of the grape varieties selected in the project. The scientific part of the meeting was rounded off by a report from the Basel Nano Imaging Lab (NI-Lab). The use of scanning electron microscopy (cryo-SEM) is the optimal method for characterising the surface quality of berries and structural differences in the overlying waxes. In this way, samples of the selected grape varieties will be collected and analysed in various vineyards in the Upper Rhine programme region.





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